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The resin was then washed with wash buffer (20 mM HEPES (pH 7.5), 100 mM NaCl, 0.05% DDM, 20 mM imidazole), and the protein sample was eluted with elution buffer (20 mM HEPES (pH 7.5), 100 mM NaCl, 0.05% DDM, 250 mM imidazole)

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