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After adding 0.4 ml of 1 M sodium citrate buffer, pH 6.0, the mixtures were incubated at 37 °C for 10 min with shaking at 125 rpm on a rotary shaker NR-3 (TAITEC) to facilitate release of bound sulfur as H 2 S gas from the aqueous phase

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MS confirmation — potwierdzenie tożsamości obu peptydów spektrometrią masową (~880 Da dla TB-500, 1419,53 Da dla BPC-157)

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