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For IP reactions with endogenous G6PD, the supernatants were pre-cleared with protein A/G-coupled agarose (Thermo Fisher Scientific), anti-G6PD antibody was added to the cell lysates and stirred at 4 °C overnight, and then protein A/G-coupled agarose were added and incubated for 2 h, followed by washing 4 times and boiling in 5X loading buffer

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133 In prostate cancer tumors, heterogeneous expressions of the PDC proteins were found and expression of PDHα, PDK1, 2, and 4 as well as PDP1 were positively correlated with androgen expression