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Tumor cells acquire Cys indirectly via peripheral glutamine using System X c − as a carrier, SLC7A11 is upregulated in one-third of TNBC cells in vivo , and limiting glutamine uptake or inhibiting System X c − activity increases intracellular ROS levels and slows TNBC metabolism (Timmerman et al., 2013)

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Given the toxicity of the intracellular form of reduced gliotoxin, we predict that it may be imported into intracellular vesicles, possibly with concomitant oxidation for storage ( 3 )